https://www.boulderbiologics.com/home daily 1.0 2026-01-26 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697187340032-SOG3WRARZPTOM8TKX2I3/shutterstock_72880636.jpg Stem Cell Therapy | Platelet-Rich Plasma | Prolotherapy https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1639511623554-63OIMG77AI7WCXZFBAOE/knee+improvement+2.jpg Stem Cell Therapy | Platelet-Rich Plasma | Prolotherapy https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1610467393516-ATOVOKZ6ZPRHHZV61SX4/IMG_4968.jpeg Stem Cell Therapy | Platelet-Rich Plasma | Prolotherapy https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1769460247749-06XRIPZ3EXWUV5ILTJ12/MSCs.jpg Stem Cell Therapy | Platelet-Rich Plasma | Prolotherapy https://www.boulderbiologics.com/dr-glowney daily 0.75 2025-05-30 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1528924637163-WF08TYCV9TO4PXS0D5LX/shutterstock_1104178004.jpg Who We Are https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1533569116995-PSYT1M511VO42S70U8KY/DSC_1737.jpg Who We Are - Jason Glowney, MD, MS https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1529536219361-OW5ZRERYPWG6RQHIANFW/jason_emily.jpg Who We Are https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/ca93556f-3dab-43e3-8171-b15bdb13a872/LRudolph2.jpg Who We Are - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.boulderbiologics.com/orthobiologics daily 0.75 2026-01-26 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188290342-MXE8TE6ZGD2K63MLDHAL/shutterstock_73807642.jpg Clinical MSC Enumeration by Flow Cytometry https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/62c87d4e-923a-4c5a-b253-0d8aac1a849a/BMA.jpg Clinical MSC Enumeration by Flow Cytometry - Make it stand out Figure 1. Flow cytometric dot plot from native bone marrow aspirate (BMA) analyzed on the Sysmex XF-1600. CD73-positive events are displayed along the vertical axis (FITC channel), while CD271-positive events are displayed along the horizontal axis (APC channel). Gating is defined by the boxed region in the upper right quadrant, representing lineage-negative, CD73⁺/CD271⁺ events consistent with MSC-enriched populations in freshly isolated marrow. https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/542e7a93-19c2-4ed1-89dc-c9dbd9dbe4fd/BMAC.jpg Clinical MSC Enumeration by Flow Cytometry - Make it stand out Figure 2. Flow cytometric dot plot from bone marrow aspirate concentrate (BMAC) following controlled centrifugation. CD73-positive events are shown on the vertical (FITC) axis and CD271-positive events on the horizontal (APC) axis. The gated box in the upper right quadrant demonstrates relative enrichment of CD73⁺/CD271⁺ events compared with native BMA, consistent with preferential recovery of MSC-enriched populations while maintaining high cell viability https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/3d26e885-0a07-48ac-8556-07aad0db6dfa/RES.jpg Clinical MSC Enumeration by Flow Cytometry - Make it stand out Figure 3. Flow cytometric dot plot from the residual (RES) fraction remaining in sterile tubes following buffy coat collection. CD73-positive events are plotted along the vertical FITC axis and CD271-positive events along the horizontal APC axis. The gated region in the upper right quadrant contains markedly fewer CD73⁺/CD271⁺ events than in BMAC, confirming the effective recovery of MSC-enriched populations into the final concentrate. https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1c1f0160-b09e-46f0-9cbe-b0a1f13f5280/Screenshot+2026-01-26+at+3.17.32%E2%80%AFPM.png Clinical MSC Enumeration by Flow Cytometry - Make it stand out Figure 4. Quantitative flow cytometric comparison of native bone marrow aspirate (BMA), bone marrow aspirate concentrate (BMAC), and the residual (RES) fraction following buffy coat recovery. Values were obtained using live-sample analysis on the Sysmex XF-1600 and reflect nucleated cell concentration, viability, and MSC-enriched population metrics. https://www.boulderbiologics.com/general-faq daily 0.75 2024-04-05 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188881651-VIAQGQX1TFQ9DNDHNYBZ/shutterstock_1239394615.jpg General FAQ https://www.boulderbiologics.com/prp-faq daily 0.75 2026-01-27 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188974870-UH7XA2WRADUR1PFQUO4G/shutterstock_1239394615.jpg PRP FAQ https://www.boulderbiologics.com/prolotherapy daily 0.75 2026-01-28 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697187776613-A7MLJHZ0U7RJKLJ34MF8/shutterstock_72880636.jpg Prolotherapy https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1537214283759-Z00RS47TKK5GJ38Q7DNN/IMG_1689.jpg Prolotherapy https://www.boulderbiologics.com/prp daily 0.75 2026-01-27 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697187635045-0K5B9J10WAYVZRN930WL/shutterstock_72880636.jpg Platelet-Rich Plasma (PRP) https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/dad8df90-95bc-47b8-bf92-576defaad698/IMG_3513.jpg Platelet-Rich Plasma (PRP) Figure A. Baseline complete blood count (CBC) prior to PRP preparation, demonstrating the patient’s starting platelet count and leukocyte/red blood cell indices. Baseline hematologic values are used to inform PRP preparation targets and document patient-specific starting conditions. https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/618e1e75-7dce-41fe-9157-62367cbeb39a/IMG_3511.jpg Platelet-Rich Plasma (PRP) - Make it stand out Figure B. Post-processing cellular analysis of the prepared PRP product, demonstrating platelet enrichment relative to baseline and documenting red blood cell and leukocyte content. PRP composition varies by formulation and indication and is clinically relevant, as PRP is not a single uniform product. https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1534424688650-541HJN9ZSXNPK3CPFGDD/PRP-SPIN.jpg Platelet-Rich Plasma (PRP) Figure C. First centrifugation step (“soft spin”) during platelet-rich plasma preparation. Whole blood is centrifuged using controlled, low-force parameters to separate platelet-rich plasma from red blood cells and the majority of leukocytes. This step allows plasma containing suspended platelets to be isolated while minimizing platelet activation and preserving cellular integrity. https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1580234040701-5PLMQLMD9FFXAJHFLCNU/Plat_Pel.jpg Platelet-Rich Plasma (PRP) Figure D. Second centrifugation step (“hard spin”) during platelet-rich plasma preparation. The platelet-rich plasma fraction undergoes higher-force centrifugation, resulting in the concentration of platelets as a visible pellet at the bottom of the conical tubes. Following this step, excess platelet-poor plasma is removed, and the platelet pellet is resuspended to achieve a PRP formulation appropriate for the clinical indication. https://www.boulderbiologics.com/stem-cell-faq daily 0.75 2026-01-27 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188931790-2L71J5W1WQT1HL0SPWPG/shutterstock_1239394615.jpg Autologous Orthobiologic Therapy FAQ https://www.boulderbiologics.com/epat daily 0.75 2024-04-05 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697187833593-LL2U71Y0SPN4JHDOT1F1/shutterstock_72880636.jpg Extracorporeal Pulse Activation Technology (EPAT) https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1536842309827-YE46C3J3I4EFS2CJDJQB/Dr+Glowney-4.jpg Extracorporeal Pulse Activation Technology (EPAT) https://www.boulderbiologics.com/patients daily 0.75 2024-04-05 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https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390500612440-PGQSIY8KL9F7T5VIXQLO/chambers-2.jpg Home Alt A better world Starts with a choice Contact Us https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390500503574-5BAPXZO9U06UV61P49UO/chambers1.jpg Home Alt Sustainability Starts with you Join Us https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390500933384-LII4SRIPHFYOUFX0UCXC/chambers-4.jpg Home Alt A better world Starts with a choice Join Us https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390504287124-HQ8GNX1JCE22V047MSQ9/chambers-8.jpg Home Alt Preserve Nature Join Us https://www.boulderbiologics.com/stem-cell-therapy-3 daily 0.75 2026-01-28 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188080299-58YI1M5HKFPKXD7CV87C/shutterstock_64247071.jpg Traumatic Brain Injury and CTE https://www.boulderbiologics.com/comprehensive-bodywork-1 daily 0.75 2026-01-28 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https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188692938-RTW3IKX85XI3S6ODK9EW/shutterstock_1131418493.jpg WOMAC Hip/Pelvis/SI Form https://www.boulderbiologics.com/knee-form daily 0.75 2024-04-05 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188639146-BV3DDUCEPEUY4CPE9CX4/shutterstock_1131418493.jpg WOMAC Knee Form https://www.boulderbiologics.com/shoulder-form daily 0.75 2023-10-13 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188726466-4LGVZLWF1LFU2P2C8P3F/shutterstock_1131418493.jpg QDASH: Shoulder Form https://www.boulderbiologics.com/prp-1 daily 0.75 2026-01-28 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697187659339-VMUJFG3RJ0OTK5JSIINQ/shutterstock_72880636.jpg PRP + Hyaluronic Acid for Knee Osteoarthritis https://www.boulderbiologics.com/fda-guidlines daily 0.75 2024-04-06 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188052669-L5XNMP80WO13F4V4CAT6/shutterstock_64247071.jpg FDA Guidelines https://www.boulderbiologics.com/mesenchymal-stem-cells-mscs daily 0.75 2024-04-05 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697188052669-L5XNMP80WO13F4V4CAT6/shutterstock_64247071.jpg Mesenchymal Stem Cells (MSCs) https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/da4176bd-5d7b-409e-92e3-989454ba6b7e/MSC1.png Mesenchymal Stem Cells (MSCs) - Make it stand out Image – Phase contrast microscopy showing a solitary spindle-shaped MSC in culture. https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/e9fa2f7e-5d25-437a-a5ed-825cf2eb1dbe/Screenshot+2024-04-04+at+1.29.33%E2%80%AFAM.png Mesenchymal Stem Cells (MSCs) - Make it stand out Table 1. – ADSC diameter by culture time. https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/af56037e-de4e-466e-951b-ece8a2946af9/Screenshot+2024-04-04+at+1.30.05%E2%80%AFAM.png Mesenchymal Stem Cells (MSCs) - Make it stand out Table 2. – Disease conditions and affected stem cell line(s): hematopoietic stem cell (HSC), mesenchymal stem cell (MSC), adipose progenitor cell (APC), skeletal muscle progenitor cell (SMPC), endothelial progenitor cell (EPC), neural stem cell (NSC), cardiac precursor cell (CPC). https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/60d842a9-3dee-4b62-85aa-faa92f5bcf93/Screenshot+2024-04-04+at+1.30.19%E2%80%AFAM.png Mesenchymal Stem Cells (MSCs) - Make it stand out Table 3. - Muthu et al. scale cohorts and outcome effectiveness versus controls. CE – cultured expanded, UC -uncultured, WMD- weighted mean difference, CI- confidence interval. https://www.boulderbiologics.com/metagenomics daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/63406e5f-1753-44bf-9c03-619a6ffacf9a/shutterstock_1897822168.jpg Metagenomics https://www.boulderbiologics.com/longevity-medications-and-supplements daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733672079732-MH6SHB37VXOV767O7TL5/Longevity+Banner.jpg Longevity Medications and Supplements https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/8e9c98ed-7a50-4e65-b905-1393acc9196f/shutterstock_2544958697.jpg Longevity Medications and Supplements https://www.boulderbiologics.com/cardiac-health-assessment daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733647637837-0DWCYJ5376E251BN8SZU/Longevity+Banner.jpg Cardiac Health Assessment https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/bea599a5-11d0-4032-bace-2a399931c98e/shutterstock_2551038131.jpg Cardiac Health Assessment https://www.boulderbiologics.com/biologics-and-longevity daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733670184708-XV6WSB7VKC7T4T7A93RT/Longevity+Banner.jpg Biologics and Longevity https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/6c4ebcc8-f3a0-441c-9fcb-dcb14af4beca/shutterstock_2169451699.jpg Biologics and Longevity https://www.boulderbiologics.com/diet daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733671615555-CSBKBBEG0FZT1NREUEHP/Longevity+Banner.jpg Diet https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/58bbfc09-7a37-4acd-85e6-e3fce0190146/diet.jpg Diet https://www.boulderbiologics.com/hormone-replacement daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733670443823-4HWKKHS7Q0KHRHTNA57K/Longevity+Banner.jpg Hormone Replacement https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/efae9a86-418a-44f3-a5c5-e86f476a365b/shutterstock_2200976087.jpg Hormone Replacement https://www.boulderbiologics.com/the-emerging-field-of-longevity-medicine daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733591080263-MEICUL07YAV539766YLN/Clocks.jpg The Emerging Field of Longevity Medicine https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/8b2ddead-86b3-48a8-9073-8b9adf67c3fa/shutterstock_2285788795.jpg The Emerging Field of Longevity Medicine - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.boulderbiologics.com/exercise-and-longevity daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733647599329-YF05SS5X7O8HTKY8NHX8/Longevity+Banner.jpg Exercise and Longevity https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/7fd693d1-4d70-440e-8838-81128dd6081e/shutterstock_2329065823.jpg Exercise and Longevity https://www.boulderbiologics.com/mitochondrial-dysfunction daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733669926839-HA2JPV39YV6J89IACOBS/Longevity+Banner.jpg Mitochondrial Dysfunction https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/ab605470-2b6f-41ee-b4b4-ccb7684e7f1b/shutterstock_2442767879.jpg Mitochondrial Dysfunction https://www.boulderbiologics.com/neurodegeneration daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733669611146-Z8RB7L24E3HYBU9RAD1B/Longevity+Banner.jpg Neurodegeneration https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/7e36f98b-5edc-4ff9-90c0-8ede5d2350b3/shutterstock_470719823.jpg Neurodegeneration https://www.boulderbiologics.com/modern-environmental-exposures daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733647397224-J9YTCTM5M4BOBZM4557V/Longevity+Banner.jpg Modern Environmental Exposures https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/792795bf-b211-4bd6-912e-086dbe078f3c/shutterstock_1416548411.jpg Modern Environmental Exposures https://www.boulderbiologics.com/aging daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733591864647-AQQZVTKYJAMPUMP0ZXIW/Clocks.jpg Aging https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/fc2d9fe5-b301-4547-aba2-578187a68cc5/shutterstock_2274077339.jpg Aging https://www.boulderbiologics.com/metabolic-syndrome daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733669359158-LKNPE6WBK69GDSOAX3Y1/Longevity+Banner.jpg Metabolic Syndrome https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/79b11a3e-2c2f-450a-8ac7-df20a7a8019b/shutterstock_376436863.jpg Metabolic Syndrome https://www.boulderbiologics.com/epigenetics daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733646257507-JEKBAA99AVDSW81OZ4BY/Longevity+Banner.jpg Epigenetics https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/bbc5ad3d-939e-4798-a601-84201125d5c5/shutterstock_2197072529.jpg Epigenetics https://www.boulderbiologics.com/cancer-prevention daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733649683320-856HE1XDUCQ46R9KCIRG/Longevity+Banner.jpg Cancer Prevention https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/93a889b2-27a2-4403-9357-ee58b0a48b0c/shutterstock_2332837273.jpg Cancer Prevention https://www.boulderbiologics.com/cancer-diagnosis-and-treatment daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733649722308-W2RLM9OOIOJT780WIKL1/Longevity+Banner.jpg Cancer Diagnosis and Treatment https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/345fbe7e-aad6-4e79-96fb-4ec56fbb3b3d/cancer+cells.jpg Cancer Diagnosis and Treatment https://www.boulderbiologics.com/sleep daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733670910625-R8A238G2HKN8Q531SDHU/Longevity+Banner.jpg Sleep https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/d7019b72-7cd6-4236-b3e2-7d6eb382938c/shutterstock_2100448963.jpg Sleep https://www.boulderbiologics.com/cardiology-biomarkers daily 0.75 2024-12-08 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1733647561889-JIS5L87WDTWIA2YIFB5S/Longevity+Banner.jpg Cardiometabolic Biomarkers https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/4f4afde4-8009-41fa-ba6b-1e58f25112d7/shutterstock_2510848529.jpg Cardiometabolic Biomarkers https://www.boulderbiologics.com/prp-proteinrich-plasma daily 0.75 2026-01-28 https://images.squarespace-cdn.com/content/v1/5b12da36fcf7fd96d1fc783e/1697187776613-A7MLJHZ0U7RJKLJ34MF8/shutterstock_72880636.jpg PRP + Protein-Rich Plasma